Technical Topics

(TT001) A compact and sensitive methylation assay enabling cost effective, accessible, and scalable blood-based early cancer detection

(TT002) A study on eber in situ hybridization with automated high-frequency ultrasonic cavitation-assisted rapid tissue processing method

(TT003) A duplex PCR and high-resolution melting analysis method to scan ALK fusions independent of fusion partners

(TT004) Automated Reverse Transcription PCR (RT-PCR) on the Hamilton STARlet Liquid Handler Integrated with a Scalable Laboratory Information Management System (LIMS) for High Throughput Molecular Testing

(TT005) A Fast Hybridization Protocol to Reduce the Turn Around Time of ctDNA Testing in Patients with Solid Tumor Malignancies

(TT006) Automated Total Nucleic Acid Extraction from Thyroid Fine Needle Aspirates Using the EZ2 RNA/miRNA Tissue/Cells Kit

(TT007) A Framework for High-Resolution Cell-Type Deconvolution and Latent Signal Detection Using DNA Methylation Profiles on CRC

(TT008) Century-scale archival sequencing of colorectal cancer through a shifting disease landscape

(TT009) A Multi-Faceted NGS Validation: Workflow Process Improvement

(TT010) Clinical validation of a digital MLPA Hereditary Cancer Panel for Copy Number Variation

(TT011) A Novel Approach Combining Blocker Displacement Amplification with Sanger Sequencing for Detecting CXCR4 Mutations in Waldenström Macroglobulinemia

(TT012) Clinical Validation of Somatic Whole Exome Sequencing on the AVITI Platform

(TT013) A Novel Library Prep Automation Workflow for Next-Generation Sequencing for Liquid Biopsy and Cancer Research

(TT014) Failed Next-Generation Sequencing of Decalcified Specimens: A Quality Improvement Investigation

(TT015) An Unusual Case of STR Analysis with Foreign Peaks

(TT016) Revisiting QC Practices in Molecular Diagnostics: A Case Study in qPCR Monitoring, SDI Trends, and Lot-to-Lot Variation

(TT017) A Robust Platform for In Situ RNA and Protein Detection Across Diverse FFPE Tissues

(TT018) Robust variant detection with optimized multimodal CGP workflow on the Trinity™ flow cell

(TT019) Advancing Translational Cancer Research: A Unified DNA and Methylation NGS Workflow for FFPE and cfDNA

(TT020) Validation of Integrated Single Nucleotide Polymorphism (SNP)-Based Sample Authentication in an In-House Next-Generation Sequencing (NGS) Oncology Panel

(TT021) Analysis of Bioinformatics Quality Metrics to Reduce Technologist Variability and Artifactual Variant Calls in a Next Generation Sequencing Assay

(TT022) From Smear to Sequence: The Critical Role of Preanalytical Factors in Validating Cytology Specimens for Hybrid Capture Next-Generation Sequencing

(TT023) Analytical validation and clinical relevance of ctDNA dynamics in a tissue-free ctDNA monitoring assay

(TT024) High-Resolution Detection of Structural Variants Associated with Leukemia Using Electronic Genome Mapping for Molecular Diagnostics

(TT025) Assessing the impact of blood collection tubes on the performance of a novel device for enrichment of circulating tumor cells

(TT026) Measles (Rubeola) Virus Reverse-transcription Real-time PCR Assay: Development and Clinical Validation on an Automated Workflow

(TT027) Assessment of cell-free DNA (cfDNA) extraction methods using spike-in material

(TT028) Performance Comparison of Illumina MiSeq and MiSeq i100 Systems: A Multi-Assay Evaluation in Hematologic Malignancy Testing

(TT029) Automated Detection of Coverage Noise in WES-Based CNA Analysis

(TT030) Representativeness of Clone Size Measurement by Using NGS-based Immunoglobulin Gene Rearrangement Test in Comparison to Flow Cytometry in Clonal Plasma Cell Disorders

(TT031) Validation of the Promega ReliaPrep FFPE RNA Extraction Kit for Yield, Purity, and Reproducibility from FFPE Tissue

(TT032) Impact of Pre-analytical Variables on PGDx Performance at a Single Academic Institution

(TT033) Automation of PGDx elio™ tissue complete on the Beckman Coulter Biomek NGeniuS System.

(TT034) cfDNA fragmentomic profiling in pre-malignant conditions – a shallow sequencing approach to capture fragment length and native termini changes.

(TT035) Clinical Laboratory Experience with AI-Guided Karyotyping and Chromosome Analysis in Pediatric Solid Tumors

(TT036) Comprehensive Genomic Profiling of Plasma Cell Neoplasms Using Hi-C Technology: A Single-Assay Approach for Detection of Structural Variants and Copy Number Alterations

(TT037) Comprehensive solid tumor profiling for limited tissue samples with supplemental amplicon sequencing

(TT038) Demonstration of increased workflow flexibility and efficiency for novel hybridization capture approach

(TT039) Development and Characterization of an Anchored PCR Next Generation Sequencing Assay for Detection of Viral Vector Integration

(TT040) Development and Evaluation of WHO International Reference Materials for Cancer Biomarkers Molecular Diagnostics

(TT041) Development of a Combined Protocol for Simultaneous Purification of DNA or RNA from FFPE Samples Using Bionano G2 FFPE DNA Kit and the Ionic System

(TT042) Development of a Multiplex ddPCR Assay for Simultaneous Absolute Quantification of Bacterial, Fungal, and Human DNA in Metagenomic Studies

(TT043) Development of a Single-Tube, 8-Color PCR Assay for Quantifying a KRAS Mutation Panel Using True Single-Molecule Counting

(TT044) Direct comparison of single-stranded vs. double-stranded probe panels for NGS target enrichment by hybridization capture

(TT045) Direct Microbial Cell-Free DNA (mcfDNA) Detection from Plasma by Nanopore Sequencing

(TT046) Enhanced Dup-Seq BCR::ABL1 NGS Assay: Accurate and Sensitive Detection of BCR::ABL1 Fusion and Mutation from Samples with Low BCR::ABL1 Copy Number

(TT047) Evaluation of Automated FFPE Extraction Chemistries for High-Throughput Molecular Testing in a Clinical Cancer Laboratory

(TT048) Exploring Divergent Trends in Hepatitis Viral Load CAP Surveys: Investigating Systematic vs. Sample-Related Errors

(TT049) Gene Fusion Detection Performance Stability of a Single Tube NGS Test for Hematological Cancers Provides High Confidence in Fusion Prevalence Across Most Heme Cancers

(TT050) Genome-wide detection of copy number alterations for cytogenetics, cell line integrity and identity using digitalMLPA

(TT051) Genome-Wide Detection of Structural Variants in Archived Sarcomas via AI-Enabled Genomic Proximity Mapping

(TT052) Harbinger Health's Technical Optimization and Assessment of xGen™ Hybridization and Wash v3 Kit Support Application for High-Throughput Targeted Methyl Sequencing

(TT054) High-throughput methyl-binding domain-based DNA enrichment and sequencing.

(TT055) Impact and Outcome of a Comprehensive Genomic Profiling Initiative among a Tertiary Care Center’s Pediatric Cancer Cohort

(TT056) Impact of DNA fragment size on targeted sequencing using hybridization capture

(TT057) Integrated Genetic and Epigenetic Profiling from the Same DNA Molecules

(TT058) Laboratory Impact from False Positivity in Norovirus Detection on the BioFire FilmArray Gastrointestinal Panel

(TT059) Leveraging Gene Expression Data to Identify IGH Fusions in Hematologic Malignancies

(TT060) Liquid Biopsy as a Reliable Alternative for Comprehensive Genomic Profiling in Advanced-Stage Colorectal Cancer with Insufficient Tissue DNA

(TT061) Load, Scan, Purify: An Automation-Ready Kit for cfDNA Purification

(TT062) Methylome Profiling of FFPET Specimens with Low Quantity and Quality

(TT063) Multiplex detection of copy number and methylation status of HR genes in high-complexity cancer samples using digitalMLPA

(TT064) Multiplex immunofluorescence (mxIF)-guided image analysis for tumor quantification for copy number assessment

(TT065) NEBNext® enzymatic solutions for DNA methylation profiling of highly damaged DNA inputs

(TT066) NGS-based chimerism test to monitor post-transplant hematological malignancy relapse

(TT067) Not So Silent Variants: Rescuing Synonymous Variants that Malfunction Beyond the Protein Code

(TT068) Optimization of a BCR-ABL1 qPCR Assay on the Roche Digital LightCycler

(TT069) Optimizing DNA Extraction from FFPE Solid Tumors: Comparison of Preanalytical and Extraction Workflow Variables and Implementation of Tumor Area Correlation for Standardized Inputs

(TT070) Overcoming Challenges of cfDNA Analysis with Optimized Preanalytical Workflows Enables Complementary Urine and Blood Liquid Biopsy Studies

(TT071) Performance Comparison of Invivoscribe’s B-cell and T-cell LymphoTrack® Assays between MiSeqTM and MiSeq i100

(TT072) Performance Comparison of the Illumina MiSeq i100 with Established Instruments for Amplicon-Based Somatic and Liquid Biopsy Sequencing Panels

(TT073) Processor-mediated PCR: Adding new dimensions to an established technique

(TT074) Quality Assurance Lessons from an Amplified ALK-Fusion-Positive Non-Small Cell Lung Cancer Contamination Event: A Case Study in Molecular Testing Troubleshooting

(TT075) Reference Standards for Long-Read Sequencing

(TT076) Single Digit Parts per Million Detection of Molecular Residual Disease Through Integrated Tumor-Informed Whole-Genome Sequencing Analysis

(TT077) SJ-Lenti-CAR – A Clinically Validated Multiplexed ddPCR Assay for Monitoring Lentiviral-Based CAR T-Cell Therapies

(TT078) SOBA-AD Assay, A Novel, Early, and Convenient Detection Tool for the Clinician: CLIA Validation of a Highly Sensitive Blood Test for Alzheimer’s Disease

(TT079) Standardizing Clinical Workflows for Cell-Free RNA Assays: High-Throughput Biofluid Profiling and Pre-Analytical Optimization

(TT080) Synopsis of Optical Genome Mapping Quality Benchmarks in 200 Clinical Cancer Samples

(TT081) The Clinical Validation and Standardization of AI-powered Tumor Content Quantification for Molecular Pathology

(TT082) The LymphoTrack Flex Assay: A High-Throughput NGS Assay for the Assessment of Clonality in Lymphoproliferative Diseases

(TT083) Tissue Fibrosis in FFPE Samples Impacts RNA Quality Assessments Used in Tissue Selection for Spatial Transcriptomics Studies

(TT084) Transcriptomic Disease Classification and Longitudinal Monitoring via Self-Collected Dried Blood Spots

(TT085) TruLAMP: A Fast, Robust and Versatile Isothermal Amplification Technology.

(TT086) Tunable hybridization capture allows for modulation of GC coverage via simple adjustments in post-capture washes

(TT087) Utilize nanoplate digital PCR (dPCR) to validate the presence and inheritance of germline copy number variants detected by SNP oligonucleotide microarray analysis

(TT088) Validation of ddPCR Assays for Sensitive Detection of Borrelia Species in Human Samples