Developing bead-formatted qPCR/RT-qPCR assays no longer requires trial-and-error. Learn about the application of a structured, Design-of-Experiments workflow to screen lyophilization-qualified buffers, tune annealing temperatures, and balance enzyme and MgCl₂ concentrations using Promega's lyo-compatible reagents resulting in a robust and reliable clinical PCR assay compliant with MIQE 2.0 and CLSI MM03/MM06 standards. Seamlessly advance into pilot-scale and full cGMP/ISO 13485 production via Promega Custom Manufacturing, with Clinical Assay Development Services (CADS) available to support capacity or expertise gaps. Leave with a defined path from concept to bead.
