Reducing time to result is critical for pathogen detection at the point of care or in the field. One of the most effective ways to accelerate testing is through direct amplification – eliminating the need for nucleic acid extraction. This session will discuss new qPCR reagent systems with exceptional inhibitor tolerance, enabling reliable amplification from crude samples such as saliva and whole blood. It will also cover recent advances in recombinase polymerase amplification (RPA), an isothermal method that supports rapid detection workflows with faster speed, greater sensitivity, and simpler primer design than LAMP. Attendees will gain insight into enzyme innovations that improve consistency, stability, and performance in both qPCR and RPA systems.
